Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of Wnt-1 and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Expressing, Flow Cytometry, Concentration Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on the Wnt-1/β-catenin signaling pathway in MNNG-stimulated WB-F344 cells. mRNA expression levels of (A) Wnt-1, β-catenin, Cyclin D1, (B) C-myc, MMP-7, Axin2 and EpCAM were detected via reverse transcription-quantitative PCR. Protein expression levels of (C) Wnt-1, β-catenin, Cyclin D1, (D) C-myc, MMP-7, Axin2 and EpCAM were detected by performing western blotting. The data of the normal group are set to 1 and data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; MMP, matrix metalloproteinase; EpCAM, epithelial cell adhesion molecule.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Concentration Assay

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on the apoptotic rate and cell cycle in MNNG-stimulated WB-F344 cells following Wnt-1 overexpression. (A) Protein expression level of Wnt-1 was detected by performing western blotting to confirm Wnt-1 overexpression in WB-F344 cells. (B) Flow cytometry was performed to detect the apoptotic rate of each group. (C) The cell cycle of each group was detected by flow cytometry. Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model; ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; NC, negative control.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Over Expression, Expressing, Western Blot, Flow Cytometry, Concentration Assay, Negative Control

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on CK-19 and AFP in MNNG-stimulated, Wnt-1-overexpression WB-F344 cells. CK-19 protein expression was (A) detected by flow cytometry and (B) quantified. (C) Concentration of AFP protein in the supernatant of each group. Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal. *P<0.01 vs. model. ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Over Expression, Expressing, Flow Cytometry, Concentration Assay

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on MNNG-stimulated WB-F344 cells following Wnt-1 overexpression. The expression of the Wnt-1 and β-catenin proteins in each group was detected by performing an immunofluorescence assay (magnification, ×400). KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; cy, cyanine.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Over Expression, Expressing, Immunofluorescence, Concentration Assay

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on the Wnt-1/β-catenin signaling pathway in MNNG-stimulated, Wnt-1-overexpression WB-F344 cells. mRNA expression levels of (A) Wnt-1, β-catenin, Cyclin D1, (B) C-myc, MMP-7, Axin2 and EpCAM were detected by reverse transcription-quantitative PCR. Protein expression levels of (C) Wnt-1, β-catenin, Cyclin D1, (D) C-myc, MMP-7, Axin2, and EpCAM detected by western blotting. The data of the normal group are set to 1 and data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model; ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; MMP, matrix metalloproteinase; EpCAM, epithelial cell adhesion molecule.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Over Expression, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Concentration Assay

Journal: Experimental & Molecular Medicine

Article Title: Effects of microRNA-135a on the epithelial–mesenchymal transition, migration and invasion of bladder cancer cells by targeting GSK3β through the Wnt/β-catenin signaling pathway

doi: 10.1038/emm.2017.239

Figure Lengend Snippet: Primer sequences of PCR

Article Snippet: Next the membrane was washed three times with TBST for 5 min on a shaking table and blocked in bags with a sealing fluid for 1 h. The membrane was again washed three times with TBST and added with rabbit anti-human vimentin (1:1000, SR0746), GSK3β (1:5000, SR5436), β-catenin (1:5000, CRM-006), cyclinD1 (1:10000, SR0617), E-cadherin (1:50, SR0609) and HRP-labeled rabbit anti-human GAPDH (1: 500, 10494-1-AP) (ProSpec-Tany Techno-Gene Ltd, Rehovot, Israel).

Techniques: